BAM Media M53: Esculin Agar
Bacteriological Analytical Manual
Esculin Agar, Modified (CDC)
|Infusion agar||40 g|
|Ferric citrate||0.5 g|
|Distilled water||1 liter|
Heat with agitation to dissolve agar. Cool to 55°C. Adjust pH to 7.0 ± 0.2. Dispense 4 ml portions to 13 × 100 mm tubes. Autoclave 15 min at 121°C. Slant tubes.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.