• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M53: Esculin Agar

January 2001

Bacteriological Analytical Manual

Esculin Agar, Modified (CDC)

Infusion agar40 g
Esculin1 g
Ferric citrate0.5 g
Distilled water1 liter


Heat with agitation to dissolve agar. Cool to 55°C. Adjust pH to 7.0 ± 0.2. Dispense 4 ml portions to 13 x 100 mm tubes. Autoclave 15 min at 121°C. Slant tubes.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.