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BAM Media M49: EC Broth

January 2001

Bacteriological Analytical Manual

EC Broth

Trypticase or tryptose20 g
Bile salts No. 31.5 g
Lactose5 g
K2HPO44 g
KH2PO41.5 g
NaCl5 g
Distilled water1 liter


Distribute 8 ml portions to 16 x 150 mm test tubes containing inverted 10 x 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.9 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.