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BAM Media M48: EB Motility Medium

January 2001

Bacteriological Analytical Manual

EB Motility Medium

Beef extract3 g
Peptone or gelysate10 g
NaCl5 g
Agar4 g
Distilled water1 liter


Heat with agitation and boil 1-2 min to dissolve agar. Dispense 8 ml portions into 16 x 150 mm screw-cap tubes. Autoclave 15 min at 121°C.
Final pH, 7.4 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.