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BAM Media M47: Earle's Balanced Salts

January 2001

Bacteriological Analytical Manual

Earle's Balanced Salts (Phenol Red-Free)

NaCl6.8 g
KCl400 mg
CaCl2·2H2O265 mg
MgSO4·7H2O200 mg
NaH2PO4·7H2O140 mg
Glucose1.0 g
NaHCO32.2 g
Distilled water1 liter


Dissolve ingredients in the water. Sterilize by filtration. Final pH, 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.