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BAM Media M46: Eagle's Minimal Essential Medium (MEME)

January 2001

Bacteriological Analytical Manual

Eagle's Minimal Essential Medium (MEME)
(with Earle's salts and nonessential amino acids)

L-Alanine8.9 mgL-Valine46.0 mg
L-Arginine HCl126.0 mgL-Tyrosine (disodium salt)52.1 mg
L-Asparagine-H2O150.0 mgD-Calcium pantothenate1.0 mg
L-Aspartic acid13.3 mgCholine chloride1.0 mg
L-Cystine.2HCl31.29 mgFolic acid1.0 mg
L-Glutamic acid14.7 mgIsoinositol2.0 mg
L-Glutamine292.0 mgNicotinamide1.0 mg
L-Glycine7.5 mgPyridoxal HCl1.0 mg
L-Histidine HCl.H2O42.0 mgRiboflavin0.1 mg
L-Isoleucine52.0 mgThiamine HCl1.0 mg
L-Leucine52.0 mgGlucose1000.0 mg
L-Lysine HCl72.5 mgCaCl2.2H2O265.0 mg
L-Methionine15.0 mgKCl400.0 mg
D-Phenylalanine32.0 mgMgSO4.7H2O200.0 mg
L-Proline11.5 mgNaCl6800.0 mg
L-Serine10.5 mgNaHCO32200.0 mg
L-Threonine48.0 mgNaH2PO4.H2O140.0 mg
L-Tryptophan10.0 mgPhenol red10.0 mg
Distilled water1 liter


Dissolve ingredients in distilled water. Sterilize by filtration. Final pH, 7.2 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.