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BAM Media M45: Duncan-Strong Sporulation Medium, Modified

January 2001

Bacteriological Analytical Manual

Duncan-Strong (DS) Sporulation Medium, Modified
(for C. perfringens)

Proteose peptone15 g
Yeast extract4 g
Sodium thioglycollate1 g
Na2HPO4.7H2O10 g
Raffinose4 g
Distilled water1 liter


Dissolve ingredients and sterilize by autoclaving for 15 min at 121°C. Adjust to pH 7.8 ± 0.1, using filter-sterilized 0.66 M sodium carbonate.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.