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U.S. Department of Health and Human Services


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BAM Media M193: Dey-Engley Broth

December 2005

Bacteriological Analytical Manual

Dey-Engley Broth

Tryptone5.0 g
Yeast extract2.5 g
Glucose10.0 g
Sodium thioglycollate1.0 g
Sodium thiosulfate6.0 g
Sodium bisulfite2.5 g
Polysorbate 805.0 g
Lecithin (soy bean)7.0 g
Brom cresol purple0.02 g
Distilled water1 L

Autoclave 15 min. at 121°C.
Final pH 7.6 ± 0.2.