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BAM Media M44: Decarboxylase Basal Medium (Arginine, Lysine, Ornithine)

January 2001

Bacteriological Analytical Manual

M44
Decarboxylase Basal Medium (Arginine, Lysine, Ornithine)

 

Base

Peptone or gelysate5 g
Yeast extract3 g
Glucose1 g
Bromcresol purple0.02 g
Distilled water1 liter

 

Adjust pH so that value after sterilization is 6.5 ± 0.2. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 10 min at 121°C. Screw the caps on tightly for storage and after inoculation. For control, use unsupplemented base.

 

For to 1 liter base, add:
Arginine Broth5 g  L-arginine
Lysine Broth (Falkow)5 g L-lysine
Ornithine Broth5 g L-ornithine

 

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.