BAM Media M43: Cooked Meat Medium (Modified)

January 2001

(a) Cooked meat medium

commercially available in dehydrated form

Beef heart 454 g
Proteose peptone 20 g
Dextrose 2 g
NaCl 5 g

(b) Diluent

(not available commercially)

Tryptone 10 g
Sodium thioglycollate 1 g
Soluble starch 1 g
Dextrose 2 g
Neutral red (1% aqueous) 5 ml
Distilled water 1 liter

Adjust pH to 6.8 ± 0.2. Add 1 g dehydrated (a) and 15 ml (b) to 20 × 150 mm tubes. Let meat particles rehydrate. Autoclave 15 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 08/05/2015
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