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U.S. Department of Health and Human Services

Food

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BAM Media M42: Cooked Meat Medium

January 2001

Bacteriological Analytical Manual

M42
Cooked Meat Medium

Beef heart454 g
Proteose peptone20 g
Dextrose2 g
NaCl5 g
Distilled water1 liter

 

Follow directions as in M38, or suspend 12.5 g commercial dehydrated cooked medium in 100 ml cold distilled water. Mix and let stand 15 min to wet particles thoroughly. Or distribute 1.25 g into 20 x 150 mm test tubes, add 10 ml cold distilled water, and mix thoroughly to wet all particles. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2. Steam the sterilized medium and cool, without agitation, just before use.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.