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BAM Media M118: Oxford Medium

January 2001

Bacteriological Analytical Manual

Oxford Medium

Columbia blood agar base39.0 g
Esculin1.0 g
Ferric ammonium citrate0.5 g
Lithium chloride15.0 g
Cycloheximide0.4 g
Colistin sulfate0.02 g
Acriflavin0.005 g
Cefotetan0.002 g
Fosfomycin0.010 g
Distilled water1 liter


Add the 55.5 g of the first 4 components (basal medium) to 1 liter distilled water. Bring gently to boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 min. Cool to 50°C and aseptically add supplement, mix, and pour into sterile petri dishes. To prepare supplement, dissolve cycloheximide, colistin sulfate, acriflavin, cefotetan, and fosfomycin in 10 ml of 1:1 mixture of ethanol and distilled water. Filter-sterilize supplement before use. Oxford basal medium and supplement mixture are available commercially.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.