Bacteriological Analytical Manual
OF Glucose Medium, Semisolid
|Tryptone (trypticase)||2 g|
|Dipotassium phosphate||0.3 g|
|Bromthymol blue dye||0.03 g|
|Distilled water||1 liter|
Boil ingredients to dissolve.
For halophilic Vibrio spp., add 15 g NaCl (2% NaCl, final concentration) to medium.
Dispense 5 ml into 13 × 100 mm tubes and autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.
*To use OF medium with sugars other than glucose, prepare medium without glucose in 900 ml water, sterilize as above, and cool to 45-50°C. Add 100 ml of 10% solution of filter-sterilized sugar to basal medium. Aseptically dispense 5 ml into sterile 13 × 100 mm tubes.
For Campylobacter, prepare half the tubes with glucose and half without.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.