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BAM Media M116: OF Glucose Medium, Semisolid

January 2001

Bacteriological Analytical Manual

OF Glucose Medium, Semisolid

Tryptone (trypticase)2 g
NaCl5 g
Dipotassium phosphate0.3 g
Bromthymol blue dye0.03 g
Agar2 g
Glucose10 g
Distilled water1 liter


Boil ingredients to dissolve.


For halophilic Vibrio spp., add 15 g NaCl (2% NaCl, final concentration) to medium.


Dispense 5 ml into 13 x 100 mm tubes and autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.


*To use OF medium with sugars other than glucose, prepare medium without glucose in 900 ml water, sterilize as above, and cool to 45-50°C. Add 100 ml of 10% solution of filter-sterilized sugar to basal medium. Aseptically dispense 5 ml into sterile 13 x 100 mm tubes.


For Campylobacter, prepare half the tubes with glucose and half without.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.