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U.S. Department of Health and Human Services


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BAM Media M115: Nutrient Gelatin (CDC)

January 2001

Bacteriological Analytical Manual

Nutrient Gelatin (CDC)
(for Gram-negative nonfermentative bacteria)

Infusion broth25 g
Gelatin120 g
Distilled water1 liter


Heat with agitation to dissolve. Cool to 55°C and adjust pH to 7.4 ± 0.2. Dispense 4 ml portions into 13 x 100 mm screw-cap tubes. Autoclave 15 min at 121°C.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.