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U.S. Department of Health and Human Services


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BAM Media M114: Nutrient Broth

January 2001

Bacteriological Analytical Manual

Nutrient Broth

Beef extract3 g
Peptone5 g
Distilled water1 liter


Heat to dissolve. Dispense 10 m1 portions into tubes or 225 ml portions into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.