BAM Media M114: Nutrient Broth

January 2001

Bacteriological Analytical Manual

Beef extract 3 g
Peptone 5 g
Distilled water 1 liter

Heat to dissolve. Dispense 10 m1 portions into tubes or 225 ml portions into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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