• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M112: Nutrient Agar

January 2001

Bacteriological Analytical Manual

Nutrient Agar

Beef extract3 g
Peptone5 g
Agar15 g
Distilled water1 liter


Heat to boiling to dissolve ingredients. Dispense into tubes or flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2. If used as base for blood agar, add 8 g NaCl to prevent hemolysis of blood cells.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.