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BAM Media M110: Nitrate Reduction Medium and Reagents

January 2001

Bacteriological Analytical Manual

M110
Nitrate Reduction Medium and Reagents


 

Culture medium

Prepare nitrate broth (M108) from nutrient broth (M114) containing 1.0 g/liter potassium nitrate.

Reagent A. Dissolve 0.5 g α-naphthylamine (a carcinogen) in 100 ml 5 N acetic acid by gently heating. Prepare 5 N acetic acid by adding distilled water to 28.7 ml glacial acetic acid (17.4 N) to give final volume of 100 ml.

Reagent B. Dissolve 0.8 g sulfanilic acid in 100 ml 5 N acetic acid by gently heating.

Reagent C. Dissolve 1 g α-naphthol in 200 ml acetic acid.

Zinc powder.

Cadmium reagent. Place zinc rods in 20% solution of cadmium sulfate for several hours. Draw off precipitated cadmium and add to 1 N HCl.


 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.