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U.S. Department of Health and Human Services


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BAM Media M108: Nitrate Broth

January 2001

Bacteriological Analytical Manual

Nitrate Broth

Beef extract3 g
Peptone5 g
KNO3 (nitrite-free)1 g
Distilled water1 liter


Dissolve ingredients. Dispense 5 ml portions into 16 x 125 mm tubes. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.