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BAM Media M107: Mueller-Hinton Agar

January 2001

Bacteriological Analytical Manual

Mueller-Hinton Agar

Beef, infusion from300 g
Acidicase Peptone (BBL)
Casamino Acids (Difco)
17.5 g
Starch1.5 g
Agar17 g
Distilled water1 liter


Heat to boiling for 1 min. Autoclave 15 min at 116°C. Final pH, 7.3 ± 0.2.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.