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U.S. Department of Health and Human Services


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BAM Media M105: Mucate Broth

January 2001

Bacteriological Analytical Manual

Mucate Broth

Peptone10 g
Mucic acid10 g
Bromthymol blue0.024 g
Distilled water1 liter


Dissolve peptone. Dissolve mucic acid by slowly adding 5 N NaOH and stirring. Dispense 5 ml portions into 13 x 100 mm screw-cap tubes. Autoclave 10 min at 121°C. Final pH, 7.4 ± 0.1.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.