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U.S. Department of Health and Human Services

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Bacteriological Analytical Manual (BAM) Media M104: MR-VP Broth

January 2001

Medium 1

Buffered peptone-water powder
(Difco or BBL)
7 g
Glucose5 g
K2HP045 g
Distilled water1 liter


 

Medium 2

Pancreatic digest of casein3.5 g
Peptic digest of animal tissue3.5 g
Dextrose5.0 g
Potassium phosphate5.0 g
Distilled water1 liter


 

Dissolve ingredients in water with gentle heat if necessary. Dispense 10 ml into 16 x 150 mm test tubes and autoclave 15 min at 118-121°C. Final pH, 6.9 ± 0.2.

 

Medium 3

Peptone5.0 g
Glucose5.0 g
Phosphate buffer5.0 g
Distilled water1 liter

 

Dissolve ingredients in water. Dispense 10 ml into 16 x 150 mm test tubes and autoclave 15 min at 121°C. Final pH, 7.5 ± 0.2.

 

For Salmonella: Dispense 10 ml into 16 x 150 mm test tubes and autoclave 12-15 min at 121°C.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.