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U.S. Department of Health and Human Services


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Bacteriological Analytical Manual (BAM) Media M103: Motility Test Medium (Semisolid)

January 2001

Beef extract 3 g
Peptone or gelysate 10 g
*NaCl 5 g
Agar 4 g
Distilled water 1 liter


General Instructions: Heat with agitation and boil 1-2 min to dissolve agar. For use with Salmonella, see instructions listed below. Dispense 8 ml portions into 16 x 150 screw-cap tubes. Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2.


*For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

For Listeria: Keep individual tubes tightly screw capped and sealed with parafilm. Store in refrigerator for up to two weeks.

For Salmonella: Dispense 20 ml portions into 20 x 150 mm screw-cap tubes, replacing caps loosely. Autoclave 15 min at 121°C. Cool to 45°C after autoclaving. Tighten caps, and refrigerate at 5-8°C. To use, remelt in boiling water or flowing steam, and cool to 45°C. Aseptically dispense 20 ml portions into sterile 15 x 100 mm petri plates. Cover plates and let solidify. Use same day as prepared. Final pH, 7.4  ± 0.2.



Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.