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BAM Media M102: Motility-Nitrate Medium, Buffered

January 2001

Bacteriological Analytical Manual

Motility-Nitrate Medium, Buffered
(for C. perfringens)

Beef extract3 g
Peptone (Difco)5 g
KNO31 g
Na2HPO32.5 g
Agar3 g
Galactose5 g
Glycerin (reagent grade)5 ml
Distilled water1 liter


Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 x 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.