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BAM Media M101: Motility Nitrate Medium

January 2001

Bacteriological Analytical Manual

Motility Nitrate Medium (for Cosmetics)
(for Gram-negative nonfermentative bacteria)

Tryptose10 g
Heart infusion agar (Difco)8 g
Potassium (or sodium) nitrate1 g
Glucose0.5 g
Distilled water1 liter


Heat with agitation to dissolve agar. Dispense 4 ml portions into 13 x 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Available as prepared medium from BBL and Scott.


NOTE: Make sure nitrates are nitrite-free.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.