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BAM Media M99: Motility-Indole-Ornithine (MIO) Medium

January 2001

Bacteriological Analytical Manual

Motility-Indole-Ornithine (MIO) Medium

Yeast extract3 g
Peptone10 g
Tryptone10 g
L-Ornithine HCl5 g
Dextrose1 g
Agar2 g
Bromcresol purple0.02 g
Distilled water1 liter


Dispense 4 ml portions into 13 x 100 mm tubes. Autoclave 15 min at 121°C. Final pH, 6.5 ± 0.2.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.