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U.S. Department of Health and Human Services

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BAM Media M93: Malt Extract Agar

January 2001

Bacteriological Analytical Manual

M93
Malt Extract Agar (Cosmetics-General Microbiology)

Malt extract30 g
Agar20 g
Distilled water1 liter

 

Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121°C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ± 0.2.

 

For cosmetics use

Cool medium to 47-50°C after autoclaving. Dispense 4 ml stock filter-sterilized chlorotetracycline HCl solution (1 g/100 ml) per liter of medium to yield final concentration of 40 ppm chlorotetracycline HCl. Mix thoroughly and dispense 20 ml portions into 15 x 100 mm petri dishes.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.