BAM Media M161: Tryptone Broth and Tryptone Salt Broths
Bacteriological Analytical Manual
Tryptone Broth and Tryptone Salt Broths
(T1N0, T1N1, T1N3, T1N6, T1N8, T1N10)
|NaCl||0, 10, 30, 60, 80, or 100 g|
|Distilled water||1 liter|
Dissolve ingredients in distilled water.
For T1N0, add no NaCl.
For T1N1, use 10 g NaCl [1% (w/v) NaCl].
For T1N3, use 30 g NaCl/liter [3% (w/v) NaCl], etc.
Dispense into 16 × 125 mm screw-cap tubes. Tighten caps to maintain correct salt concentration in tube. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.