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BAM Media M160: Trypticase Soy-Tryptose Broth

January 2001

Bacteriological Analytical Manual

Trypticase Soy-Tryptose Broth

Trypticase soy broth
(commercial, dehydrated)
15 g
Tryptose broth
(commercial, dehydrated)
13.5 g
Yeast extract3 g
Distilled water1 liter


Dissolve ingredients in 1 liter water. Heat gently to dissolve. Dispense 5 ml portions into 16 x 150 mm test tubes. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.