• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M157: Trypticase Soy Broth

January 2001

Bacteriological Analytical Manual

Trypticase Soy Broth with 0.6% Yeast Extract (TSBYE)

Trypticase soy broth30 g
Yeast extract6 g
Distilled water1 liter


Weigh ingredients and dissolve in water. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.