Bacteriological Analytical Manual
|Trypticase or tryptose
(pancreatic digest of casein)
(papaic digest of soya meal)
|Sodium pyruvate||10 g|
|Distilled water||1 liter|
Dehydrated trypticase or tryptic soy broth is satisfactory with 95 g NaCl and 10 g sodium pyruvate added per liter. Adjust to pH 7.3. Heat gently if necessary. Dispense 10 ml into 16 × 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2. Store up to 1 month at 4 ± 1°C.
|Temperature||4 ± 1° C|
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.