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U.S. Department of Health and Human Services


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BAM Media M154a: Trypticase (Tryptic) Soy Broth

January 2001

Bacteriological Analytical Manual

Trypticase (Tryptic) Soy Broth with 10% NaCl and 1% Sodium Pyruvate

Trypticase or tryptose
(pancreatic digest of casein)
17 g
(papaic digest of soya meal)
3 g
NaCl100 g
K2HPO42.5 g
Dextrose2.5 g
Sodium pyruvate10 g
Distilled water1 liter


Dehydrated trypticase or tryptic soy broth is satisfactory with 95 g NaCl and 10 g sodium pyruvate added per liter. Adjust to pH 7.3. Heat gently if necessary. Dispense 10 ml into 16 x 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2. Store up to 1 month at 4 ± 1°C.


Temperature4 ± 1° C
Duration1 month


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.