Bacteriological Analytical Manual
Trypticase (Tryptic) Soy Broth (TSB) with Glycerol
|Trypticase peptone (tryptone)||17 g|
|Phytone peptone (soytone)||3 g|
|Distilled water||760 ml|
Suspend ingredients in distilled water and heat to dissolve. Dispense into bottles or vials. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.