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BAM Media M186: Trypicase (Tryptic) Soy Broth with ferrous sulfate

January 2001

Bacteriological Analytical Manual

Trypicase (Tryptic) Soy Broth with ferrous sulfate

Trypticase peptone17 g
Phytone peptone3 g
NaCl5 g
K2HPO42.5 g
Glucose2.5 g
Ferrous sulfate35 mg
Distilled water1.0 liter


Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.


Hypertext Source: adapted from Chapter 5, Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Created 2000-MAR-01