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BAM Media M156: Trypticase Soy Broth Modified (mTSB)

January 2002

Bacteriological Analytical Manual

Trypticase Soy Broth Modified

Trypticase soy broth30.0 g
Bile salts No. 31.5 g
Dipotassium phosphate1.5 g
Novobiocin solution (R50)0.2 ml
Deionized water1 liter


Autoclave mTSB without novobiocin and let cool to room temperature. Final pH, 7.4 +/- 0.2. For chapter 24, gene probe method, add novobiocin just before adding food.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Revisions: January 28, 2002 Required pH specified.