BAM Media M152a: Trypticase Soy Agar-Magnesium sulfate-NaCl (TSAMS)

May 2004

Bacteriological Analytical Manual

Trypticase peptone 15 g
Phytone peptone 5 g
NaCl 20 g
MgSO4·7H2O 1.5 g
Agar 15 g
Distilled water 1 liter

Heat with agitation to dissolve agar. Boil 1 min. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.

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