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BAM Media M152: Trypticase (Tryptic) Soy Agar

January 2001

Bacteriological Analytical Manual

Trypticase (Tryptic) Soy Agar

Trypticase peptone15 g
Phytone peptone5 g
NaCl5 g
Agar15 g
Distilled water1 liter


Heat with agitation to dissolve agar. Boil 1 min. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.