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BAM Media M151: Trypticase-Peptone-Glucose-Yeast Extract Broth (TPGY)

January 2001

Bacteriological Analytical Manual

Trypticase-Peptone-Glucose-Yeast Extract Broth

Trypticase50 g
Peptone5 g
Yeast extract20 g
Dextrose4 g
Sodium thioglycollate1 g
Distilled waterl liter


Dissolve solid ingredients and dispense 15 ml in 20 x 150 mm tubes. Autoclave tubes 10 min at 121°C. Final pH, 7.0 ± 0.2. Refrigerate at 5°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.