• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M150: Trypticase Novobiocin (TN) Broth

January 2001

Bacteriological Analytical Manual

M150 Trypticase Novobiocin (TN) Broth

Trypticase soy broth30 g
Bile salts No. 31.5 g
Dipotassium phosphate1.5 g
Novobiocin20 mg
Distilled water1 liter


Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize.

Make fresh stock each time of use, or store frozen at -10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.

Make up fresh
Storage:Frozen [-10°C]
dark conditions
up to 1 month


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.