Bacteriological Analytical Manual
|Polypeptone||20 g||Beef extract||3 g|
|NaCl||5 g||Yeast extract||3 g|
|Lactose||10 g||Peptone||15 g|
|Sucrose||10 g||Proteose peptone||5 g|
|Glucose||1 g||Glucose||1 g|
|Fe(NH4)2(SO4)2•6H2O||0.2 g||Lactose||10 g|
|Na2S2O3||0.2 g||Sucrose||10 g|
|Phenol red||0.025 g||FeSO4||0.2 g|
|Agar||13 g||NaCl||5 g|
|Distilled water||1 liter||Na2S2O3||0.3 g|
|Phenol red||0.024 g|
|Distilled water||1 liter|
These two media are interchangeable for general use.
Suspend ingredients of Medium 1 in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min to dissolve ingredients. Fill 16 × 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave Medium 1 for 15 min at 118°C.
Prepare Medium 2 in the same manner as Medium 1, except autoclave 15 min at 121°C. Before the media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt. Final pH, 7.3 ± 0.2 for Medium 1 and 7.4 ± 0.2 for Medium 2.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.