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BAM Media M148: Toluidine Blue-DNA Agar

January 2001

Bacteriological Analytical Manual

M148 Toluidine Blue-DNA Agar


Deoxyribonucleic acid (DNA)0.3 g
Agar10 g
CaCl2 (anhydrous)1.1 mg
NaCl10 g
Toluidine blue O0.083 g
Tris (hydroxymethyl) aminomethane6.1 g
Distilled water1 liter


Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water. Adjust pH to 9.0. Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve. Dissolve toluidine blue O in medium. Dispense to rubber-stoppered flasks. Sterilization is not necessary if used immediately. The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.


Storageroom temperature
Shelf life4 months


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.