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BAM Media M147: Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar

January 2001

Bacteriological Analytical Manual

Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar

Yeast extract5 gOxgall5 g
Peptone10 gNaCl10 g
Sucrose20 gFerric citrate1 g
Sodium thiosulfate 5H2O10 gBromthymol blue0.04 g
Sodium citrate 2H2O10 gThymol blue0.04 g
Sodium cholate3 gAgar15 g
Distilled water1 liter


Prepare in flask at least 3 times larger than required volume of medium. Add ingredients to warm distilled water and heat to dissolve. Bring just to boil, and immediately remove from heat. DO NOT AUTOCLAVE! Cool to 50°C and pour into petri dishes. Dry the plates overnight or at 37-45°C before use.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.