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BAM Media M145: Tetrathionate Broth

January 2001

Bacteriological Analytical Manual

M145 Tetrathionate (TT) Broth

 

Tetrathionate broth base

Polypeptone5 g
Bile salts1 g
Calcium carbonate10 g
Sodium thiosulfate.5H2O30 g
Distilled water1 liter

 

Suspend ingredients in 1 liter distilled water, mix, and heat to boiling. DO NOT AUTOCLAVE. (Precipitate will not dissolve completely.) Cool to less than 45°C. Store at 5-8°C. Final pH, 8.4 ± 0.2.

  

Iodine-Potassium Iodide (I2-KI) solution

Potassium iodide5 g
Iodine, resublimed6 g
Distilled water, sterile20 ml

 

Dissolve potassium iodide in 5 ml sterile distilled water. Add iodine and stir to dissolve. Dilute to 20 ml. 

 

Brilliant green solution

Brilliant green dye, sterile0.1 g
Distilled water, sterile100 m1

 

On day of use, add 20 ml I2-KI solution and 10 ml brilliant green solution to 1 liter base. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 x 150 or 16 x 150 mm sterile test tubes. Do not heat medium after addition of I2-KI and dye solutions. 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.