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U.S. Department of Health and Human Services


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BAM Media M144: T1N1 Medium

January 2001

Bacteriological Analytical Manual

T1N1 Medium

Trypticase10 g
NaCl10 g
Agar (if solid medium is preferred)20 g
Distilled water1 liter


Heat if necessary to dissolve ingredients. Dispense into 16 x 125 mm screw-cap tubes (if tubed medium is required). Autoclave 15 min at 121°C. Slant tubes until cool or let medium cool to 50 °C and pour into 15 x 100 mm petri dishes. Final pH, 7.1 ± 0.2.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.