BAM Media M141: Spray's Fermentation Medium
Bacteriological Analytical Manual
Spray's Fermentation Medium
(for Clostridium perfringens)
|Sodium thioglycollate||0.25 g|
Dissolve all ingredients except agar and adjust pH to 7.4 ± 0.2. Add agar and heat with agitation to dissolve. Dispense 9 ml portions into 16 × 125 mm tubes. Autoclave 15 min at 121 °C. Before use, heat in boiling water or flowing steam for 10 min. Add 1 ml of sterile 10% carbohydrate solution to 9 ml base.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.