• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services


  • Print
  • Share
  • E-mail

BAM Media M140: Sporulation Broth

January 2001

Bacteriological Analytical Manual

Sporulation Broth
(for Clostridium perfringens)

Polypeptone15 g
Yeast extract3 g
Starch, soluble3 g
MgSO4 (anhydrous)0.1 g
Sodium thioglycollate1 g
Na2HPO411 g
Distilled water1 liter


Adjust pH to 7.8 ± 0.1. Dispense 15 ml portions into 20 x 150 mm screw-cap tubes. Autoclave 15 min at 121°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.