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U.S. Department of Health and Human Services


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BAM Media M139: Sorbitol-MacConkey Agar

January 2001

Bacteriological Analytical Manual

Sorbitol-MacConkey Agar

Peptone or gelysate17.0 g
Protease peptone No. 3 or polypeptone3.0 g
Sorbitol10.0 g
Bile salts, purified1.5 g
NaCl5.0 g
Agar13.5 g
Neutral red0.03 g
Crystal violet0.001 g
Distilled water1 liter


Dissolve ingredients in distilled water by heating with stirring. Autoclave 15 min at 121°C. Final pH, 7.1 ± 0.2.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.