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U.S. Department of Health and Human Services

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BAM Media M136: Shigella Broth

January 2001

Bacteriological Analytical Manual

M136
Shigella Broth

 

Base

Tryptone20 g
K2HPO42 g
KH2PO42 g
NaCl5 g
Glucose1 g
Tween 801.5 ml
Distilled water1 liter

 

Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

 

Novobiocin solution

Weigh 50 mg novobiocin into 1 liter distilled water.

Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.

 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.