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BAM Media M133: Sabouraud's Dextrose Broth and Agar

January 2001

Bacteriological Analytical Manual

Sabouraud's Dextrose Broth and Agar

Polypeptone or neopeptone10 g
Dextrose40 g
Distilled water1 liter


Dissolve completely and dispense 40 ml portions into screw-cap bottles. Final pH, 5.8. Autoclave 15 min at 118-121°C. Do not exceed 121°C.


For Sabouraud's dextrose agar, prepare broth as above and add 15-20 g agar, depending on gel strength desired. Final pH, 5.6 ± 0.2. Dispense into tubes for slants and bottles or flasks for pouring plates. Autoclave 15 min at 118-121 °C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.