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BAM Media M130: Purple Carbohydrate Fermentation Broth Base

January 2001

Bacteriological Analytical Manual

Purple Carbohydrate Broth

Proteose peptone No. 310 g
Beef extract1 g
NaCl5 g
Bromcresol purple0.02 g
Distilled water1 liter


Prepare as for phenol red carbohydrate broth (M121).


Final pH, 6.8 ± 0.2.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.