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BAM Media M128: Pseudomonas Agar F

January 2001

Bacteriological Analytical Manual

Pseudomonas Agar F
(for fluorescein production)


Use Difco product, if available.


Proteose peptone No. 3 (Difco)20.0 g
Tryptone (Difco)10.0 g
Dipotassium phosphate1.5 g
Magnesium sulfate0.73 g
Glycerol (Difco)10.0 g
Agar15.0 g
Distilled water1 liter


Add medium powder and glycerol to water; mix. Heat to boiling to dissolve ingredients. Autoclave at 121°C for 15 min. Final pH, 7.0.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.