BAM Media M128: Pseudomonas Agar F

January 2001

Bacteriological Analytical Manual

Pseudomonas Agar F
(for fluorescein production)

Use Difco product, if available.

Proteose peptone No. 3 (Difco) 20.0 g
Tryptone (Difco) 10.0 g
Dipotassium phosphate 1.5 g
Magnesium sulfate 0.73 g
Glycerol (Difco) 10.0 g
Agar 15.0 g
Distilled water 1 liter

Add medium powder and glycerol to water; mix. Heat to boiling to dissolve ingredients. Autoclave at 121°C for 15 min. Final pH, 7.0.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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