BAM Media M124: Plate Count Agar (Standard Methods)

January 2001

Bacteriological Analytical Manual

Tryptone 5 g
Yeast extract 2.5 g
Dextrose 1 g
Agar 15 g
Distilled water 1 liter

Heat to dissolve ingredients. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

For viable yeasts and molds, dispense 20-25 ml portions into sterile 15 × 100 mm petri dishes.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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