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U.S. Department of Health and Human Services

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BAM Media M124: Plate Count Agar

January 2001

Bacteriological Analytical Manual

M124
Plate Count Agar
(Standard Methods)

Tryptone5 g
Yeast extract2.5 g
Dextrose1 g
Agar15 g
Distilled water1 liter

 

Heat to dissolve ingredients. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

 

For viable yeasts and molds, dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes.

 


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.