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BAM Media M122: Phenol Red Glucose Broth

January 2001

Bacteriological Analytical Manual

Phenol Red Glucose Broth

Proteose peptone No. 310 g
NaCl5 g
Beef extract (optional)1 g
Dextrose5 g
Phenol red
(7.2 ml of 0.25% solution)
0.018 g
Distilled water1 liter


Dispense 2.5 ml portions into 13 x 100 mm tubes. Autoclave 10 min at 118°C. Final pH, 7.4 ± 0.2.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.